Related topics: Protein tags
See list of additional Strep-tag antibodies at Linscott’s Directory
On January 5, 2017 Seandean Harwood, Aarhus University wrote:
StrepMab Classic Cat.no: 2-1507-001 from Iba
I have used this antibody for detecting StrepII-tagged recombinant proteins in several different assays, where it has performed without issue.
ELISA: used at 0.5 ug/mL in 2% BSA PBS, followed by detection with a GAM-HRP
Western blot: used at 0.25 ug/mL in 2% milk powder – PBS, then detected with GAM-HRP
FACS: used at 5 ug/mL in 1% FBS PBS, then detected with GAM-PE.
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On January 5, 2017 Seandean Harwood, Aarhus University wrote:
StrepMab Classic
Cat.no: 2-1507-001 from Iba
I have used this antibody for detecting StrepII-tagged recombinant proteins in several different assays, where it has performed without issue.
ELISA: used at 0.5 ug/mL in 2% BSA PBS, followed by detection with a GAM-HRP
Western blot: used at 0.25 ug/mL in 2% milk powder – PBS, then detected with GAM-HRP
FACS: used at 5 ug/mL in 1% FBS PBS, then detected with GAM-PE.