On January 5, 2017Seandean Harwood, Aarhus University wrote:
5
Goat anti-mouse IgG (Fc specific) HRP conjugate
A2554 from Sigma
This is a standard antibody widely used in sandwich detection with murine antibodies at my lab (e.g. using 9E10 anti-myc, M2 anti-FLAG, StrepMab Classic anti-StrepII etc.).
On January 5, 2017 Seandean Harwood, Aarhus University wrote:
Goat anti-mouse IgG (Fc specific) HRP conjugate
A2554 from Sigma
This is a standard antibody widely used in sandwich detection with murine antibodies at my lab (e.g. using 9E10 anti-myc, M2 anti-FLAG, StrepMab Classic anti-StrepII etc.).
Western blot: Used 1:10000 in 2% milk powder PBS
ELISA: Used 1:1000 in 2% BSA PBS.