On January 5, 2017Seandean Harwood, Aarhus University wrote:
5
rat anti-mouse CD31 primary antibody
(553370, BD Pharmingen)
I used this antibody to visualize murine CD31 in tissues taken from mice (fixed with 4% formaldehyde and frozen in OCT) in immunofluorescence microscopy. The vasculature was brightly visible, consistently.
Used diluted 200x in 1% BSA FBS, detected with a donkey anti-rat Cy3 conjugate.
On January 5, 2017 Seandean Harwood, Aarhus University wrote:
rat anti-mouse CD31 primary antibody
(553370, BD Pharmingen)
I used this antibody to visualize murine CD31 in tissues taken from mice (fixed with 4% formaldehyde and frozen in OCT) in immunofluorescence microscopy. The vasculature was brightly visible, consistently.
Used diluted 200x in 1% BSA FBS, detected with a donkey anti-rat Cy3 conjugate.