Works fine for ICC of hiPSC derived neuronal cultures/ cerebral organoids.
Dilution used: 1:100
For more information see Ghatak et al. eLife 2019, “Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs. isogenic controls“ https://elifesciences.org/articles/50333v1
This antibody works roughly for immunohistochemistry.
Dilution used: 1:1000
For more information see Kumamaru et al. Cell Reports 2019, “Regenerating Corticospinal Axons Innervate Phenotypically Appropriate Neurons within Neural Stem Cell Grafts”
On January 5, 2017Mohammad Baragji, University of Copenhagen wrote:
5
Single staining using an antibody raised towards GABA.
Primary antibody: Anti-GABA antibody produced in rabbit [Sigma, cat: A2052]
Secondary antibody: Goat anti Rabbit HRP-conjugated [DAKO, cat: P0448]
Protocol in short:
Sections were washed (3x10min), endogenous peroxidase blocked, treated with target retrieval solution and washed again (3×10 min) followed by incubation with a rabbit antibody directed towards GABA (1:250) (SIGMA-ALDRICH) overnight. The following day, sections were washed (3×10 min) and incubated for 1 hour with secondary goat anti rabbit HPRT conjugated antibody (1:400) (DAKO). Sections were washed (3×10 min) and HPRT detected by adding DAB substrate solution. Sections were then washed (3×10 min) and mounted on slides, rehydrated, counterstained with hematoxylin, dehydrated and mounted with cover slips.
Results:
Staining appears specific, showing that the used antibody may be appropriate for staining GABA as a proxy marker for PV expressing interneurons in a double staining setup.
On May 18, 2020 Swagata Ghatak, The Scripps Research Institute wrote:
Works fine for ICC of hiPSC derived neuronal cultures/ cerebral organoids.
Dilution used: 1:100
For more information see Ghatak et al. eLife 2019, “Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs. isogenic controls“
https://elifesciences.org/articles/50333v1
On April 27, 2020 Moshe Parnas, Tel Aviv University wrote:
Great performance. Dilution used: 1:100
For more information see Rozenfeld et al. Cell Reports 2019, “ Muscarinic Modulation of Antennal Lobe GABAergic Local Neurons Shapes Odor Coding and Behavior”
https://www.sciencedirect.com/science/article/pii/S2211124719314585#sec5
On January 28, 2020 Hiromi Kumamaru, University of California wrote:
This antibody works roughly for immunohistochemistry.
Dilution used: 1:1000
For more information see Kumamaru et al. Cell Reports 2019, “Regenerating Corticospinal Axons Innervate Phenotypically Appropriate Neurons within Neural Stem Cell Grafts”
https://www.sciencedirect.com/science/article/pii/S2211124719301366#sec4
On January 5, 2017 Mohammad Baragji, University of Copenhagen wrote:
Single staining using an antibody raised towards GABA.
Primary antibody: Anti-GABA antibody produced in rabbit [Sigma, cat: A2052]
Secondary antibody: Goat anti Rabbit HRP-conjugated [DAKO, cat: P0448]
Protocol in short:
Sections were washed (3x10min), endogenous peroxidase blocked, treated with target retrieval solution and washed again (3×10 min) followed by incubation with a rabbit antibody directed towards GABA (1:250) (SIGMA-ALDRICH) overnight. The following day, sections were washed (3×10 min) and incubated for 1 hour with secondary goat anti rabbit HPRT conjugated antibody (1:400) (DAKO). Sections were washed (3×10 min) and HPRT detected by adding DAB substrate solution. Sections were then washed (3×10 min) and mounted on slides, rehydrated, counterstained with hematoxylin, dehydrated and mounted with cover slips.
Results:
Staining appears specific, showing that the used antibody may be appropriate for staining GABA as a proxy marker for PV expressing interneurons in a double staining setup.