In pBEC the antibody produced a single clear band at approximately 62 kDa and a few weaker bands.
In HEK 293 lysate a very weak band were formed at 62 kDa and a weak at 55 kDa.
In rAstro, a clear band was also formed at approximately 62 kDa, along with a double band around 55 kDa
On September 9, 2016 Niels M Kristiansen, Aarhus University wrote:
Ab Name: Transferrin Receptor
Producer: Bioss Antibodies
Catalog No: BS-0988R
Host organism: Rabbit
Used dilution: 1:1000 (WB) – 0.1 ug/ml
Secondary Ab: Anti-Rabbit HRP, Cell Signaling: 7074 (1:2000)
Cell line: primary porcine brain endothelial cells (pBEC), HEK 293, primary rat astrocyttes (rAstro) – 5 ug
In pBEC the antibody produced a single clear band at approximately 62 kDa and a few weaker bands.
In HEK 293 lysate a very weak band were formed at 62 kDa and a weak at 55 kDa.
In rAstro, a clear band was also formed at approximately 62 kDa, along with a double band around 55 kDa