Ab Name: His-probe (H-15)
Producer: Santa Cruz
Catalog No:sc-803
Host organism: Rabbit
Used dilution: 1:1000 (WB).
Secondary Ab: Goat anti-rabbit HRP (1:10.000)
I used the antibody for western blotting of purified his-tagged protein overexpressed in HEK293T cells. It gave nice clear bands, with some endogenous (off target) protein bands also showing up.
On September 9, 2016 Jane Hübertz Frederiksen, University of Copenhagen wrote:
Ab Name: His-probe (H-15)
Producer: Santa Cruz
Catalog No:sc-803
Host organism: Rabbit
Used dilution: 1:1000 (WB).
Secondary Ab: Goat anti-rabbit HRP (1:10.000)
I used the antibody for western blotting of purified his-tagged protein overexpressed in HEK293T cells. It gave nice clear bands, with some endogenous (off target) protein bands also showing up.