On July 13, 2016Paula Szalai, University of Oslo wrote:
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Caspase-9 Cell Signaling Technology #9502
Nice antibody that detects full-length (47 kDa) and cleaved Caspase-9 (35, 37 kDa), even though I see only one band of cleaved and not two. One unspecific band above 50, confirmed with RNAi knockdown.
1:1000 in TBS Tween 5% BSA, can be reused. Tested in cell lines (LNCaP)
On July 13, 2016 Paula Szalai, University of Oslo wrote:
Caspase-9 Cell Signaling Technology #9502
Nice antibody that detects full-length (47 kDa) and cleaved Caspase-9 (35, 37 kDa), even though I see only one band of cleaved and not two. One unspecific band above 50, confirmed with RNAi knockdown.
1:1000 in TBS Tween 5% BSA, can be reused. Tested in cell lines (LNCaP)
Please see image above