Phospho-FRS2-α (Tyr196), rabbit pAb, Cell Signaling Technology, #3864

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2 User Reviews

  • I used this antibody on murine hippocampal lysates +/- BDNF with some succes. There is a very weak band just below the .100 kDa mark in the stimulated lane. This is however barely visible (se picture) and also somewhat present in the unstimulated lane.
    Konc: 1:1000
    Exposure-time: 30 min
    lane-load: 50 ug

    Western blot33333
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Phospho-FRS2-α (Tyr196) Antibody #3864

    I used this antibody for western blotting on primary hippocampal neurons from P0 mice. I experimented with many different dilutions – 1:2000, 1:1000, 1:500 and 1:250, none of which were succesful. I suspect I may have received a defective batch of this antibody

    Western blot11111
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex
EM