There was no signal present in murine hippocampal neurons stimulated with BDNF, with a viable stimulation-control of downstream kinases (ERK and MEK).
Konc: 1:1000
Exposure: 30 min
Lan-load: 50 ug
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On July 13, 2016Signe Petersen, Aarhus University wrote:
I used this antibody for western blotting on primary hippocampal neurons from P0 mice. I experimented with many different dilutions – 1:2000, 1:1000, 1:500 and 1:250, none of which were succesful. I suspect I may have received a defective batch of this antibody.
On January 23, 2017 lars meinertz byg wrote:
There was no signal present in murine hippocampal neurons stimulated with BDNF, with a viable stimulation-control of downstream kinases (ERK and MEK).
Konc: 1:1000
Exposure: 30 min
Lan-load: 50 ug
On July 13, 2016 Signe Petersen, Aarhus University wrote:
Phospho-FRS2-α (Tyr436) Antibody #3861, Cell Signaling Technologies
I used this antibody for western blotting on primary hippocampal neurons from P0 mice. I experimented with many different dilutions – 1:2000, 1:1000, 1:500 and 1:250, none of which were succesful. I suspect I may have received a defective batch of this antibody.