Good signal in western blot. Recognizes the full length and cleaved form of the protein. Dilution 1:1000 in 1% milk in PBS-T
For more information see Rizzotto et al. Cell Reports 2020, “Nutlin-Induced Apoptosis Is Specified by a Translation Program Regulated by PCBP2 and DHX30”. https://doi.org/10.1016/j.celrep.2020.03.011
Short protocol:
1:2000 dilution in 5% low fat milk powder in PBS-T and incubate overnight at 4°C.
Review: Continuously performs very well. I kept the diluted antibody at -20° and reused it up to 5 times. Run the gel appropriately to ensure complete separation of the larger cleaved fragment (89kDa) and uncleaved PARP (116kDa).
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On July 13, 2016Paula Szalai, University of Oslo wrote:
5
PARP Cell Signaling Technology rabbit #9542
Works very well, strong specific signal. Used in cell lines (LNCaP, PC3)
Detects full-lenght at 116 kDa and cleaved at 89 kDa, apoptotic signal.
Use 1:1000 TBS Tween 5% BSA, can be reused often and also be refreshed when it is getting weak.
On April 21, 2020 Dario Rizzotto wrote:
Good signal in western blot. Recognizes the full length and cleaved form of the protein. Dilution 1:1000 in 1% milk in PBS-T
For more information see Rizzotto et al. Cell Reports 2020, “Nutlin-Induced Apoptosis Is Specified by a Translation Program Regulated by PCBP2 and DHX30”. https://doi.org/10.1016/j.celrep.2020.03.011
On October 29, 2017 Heidi Gytz Olesen, McGill University, Canada wrote:
Rabbit anti-PARP, Cell Signalling #9542
Short protocol:
1:2000 dilution in 5% low fat milk powder in PBS-T and incubate overnight at 4°C.
Review: Continuously performs very well. I kept the diluted antibody at -20° and reused it up to 5 times. Run the gel appropriately to ensure complete separation of the larger cleaved fragment (89kDa) and uncleaved PARP (116kDa).
On July 13, 2016 Paula Szalai, University of Oslo wrote:
PARP Cell Signaling Technology rabbit #9542
Works very well, strong specific signal. Used in cell lines (LNCaP, PC3)
Detects full-lenght at 116 kDa and cleaved at 89 kDa, apoptotic signal.
Use 1:1000 TBS Tween 5% BSA, can be reused often and also be refreshed when it is getting weak.
Please see image above