On January 5, 2017Seandean Harwood, Aarhus University wrote:
5
anti-HLA CLass I mAb, clone W6/32
Sigma
Used at 5 uL/million cells, detection with GAM-PE
This antibody is used at my lab to detect human MHC class I, as a positive control (especially on cell lines which are being used as negative controls for the experiment at hand). We switched to this from an anti-MHC Class I already directly conjugated to PE, as this one is more comparable to how our samples are typically analyzed (with an initial murine anti-whatever, followed by GAM-PE).
Western blot
Not Rated
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Luminex
Not Rated
EM
Not Rated
On July 13, 2016Kasper Mølgaard Jensen, Aarhus University wrote:
5
Monoclonal Anti-HLA Class I Antigen antibody produced in mouse, clone W6/32, H1650 Sigma-Aldrich
Used 5µL to stain 10^6 human CEA-positive MKN45 gastric carcinoma cells for 30 minutes prior analysis.
Very good results alone with GAM-PE pAb.
On January 5, 2017 Seandean Harwood, Aarhus University wrote:
anti-HLA CLass I mAb, clone W6/32
Sigma
Used at 5 uL/million cells, detection with GAM-PE
This antibody is used at my lab to detect human MHC class I, as a positive control (especially on cell lines which are being used as negative controls for the experiment at hand). We switched to this from an anti-MHC Class I already directly conjugated to PE, as this one is more comparable to how our samples are typically analyzed (with an initial murine anti-whatever, followed by GAM-PE).
On July 13, 2016 Kasper Mølgaard Jensen, Aarhus University wrote:
Monoclonal Anti-HLA Class I Antigen antibody produced in mouse, clone W6/32, H1650 Sigma-Aldrich
Used 5µL to stain 10^6 human CEA-positive MKN45 gastric carcinoma cells for 30 minutes prior analysis.
Very good results alone with GAM-PE pAb.