Related topics: Secondary antibodies
On January 5, 2017
Seandean Harwood, Aarhus University wrote:
AffiniPure F(ab’)₂ Fragment Goat Anti-Mouse IgG (H+L) – phycoerythrin
This antibody is widely used at my laboratory in FACS for sandwich detection, using various mouse antibodies (e.g. anti-myc, anti-FLAG, anti-StrepII), and then detecting with this GAM-PE. We use it diluted 200x in 1% FBS PBS. When detecting abundant antigens (e.g. EGFR on HeLa cells, heterogenously expressed surface receptors in HEK293 cells under CMV promoter control), we get MFI shifts around 50x.
On July 13, 2016
Kasper Mølgaard Jensen, Aarhus University wrote:
R-Phycoerythrin AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG, Fcy Fragment Specific, 115-116-071, Jackson ImmunoResearch
Used for flow cytometry analysis at working concentration of 1:200 for staining 10^6 cells.
Great results with a variety of different primary mAb.