β-Tubulin Isotype III, mouse mAb clone SDL.3D10, Sigma, T5076

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  • Sigma # T5076: mouse-anti-β-III-tub

    The antibody was used for Immunofluorescence staining of neurons derived from murine ES cells differentiated in culture.
    The cells were grown on coated cover slips, fixed for 30min in 4% PFA and treated for 5 min with 0,005% Triton-X-100. To reduce unspecific binding cells are blocked/ quenched with a 1% BSA in PBS solution.
    The Antibody was diluted 1:8000. The secondary antibody was a goat anti mouse 488 diluted 1:1000.
    Strong staining of the neurons was observed.
    In double staining experiments of the same cell lines nearly no β-III-tub could be observed when mouse-anti-β-III-tub is used together with Millipore # AB152 rabbit-anti-TH (1:500) or Sigma # A2052 rabbit-anti-GABA (1:250).

    Please see inserted pictures with the staining of Sigma # T5076 mouse-anti-β-III-tub The pictures show the differentiated wildtype cells from the ES cell line CJ7.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex
EM