I have used this antibody for ICC of primary rat and mouse Schwann cells (1:400).
Rat Schwann cells show strong staining of the nucleus and weaker staining of the cytoplasm. Mouse Schwann cells show strong staining of the cytoplasm and weaker staining of the nucleus.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 14, 2014Mads Kjolby, Aarhus University wrote:
4.5
I have used DAKO (20311) anti-s100 to test primary cultures of rat Schwann Cells for purity, I have used the antibody for western blotting (1:1000) and in for immunofluorescence in primary rat Schwann cells. It work very well and is very sensitive for western blotting. Staining also looks correct.
On May 28, 2014 Maj Ulrichsen wrote:
I have used this antibody for ICC of primary rat and mouse Schwann cells (1:400).
Rat Schwann cells show strong staining of the nucleus and weaker staining of the cytoplasm. Mouse Schwann cells show strong staining of the cytoplasm and weaker staining of the nucleus.
On February 14, 2014 Mads Kjolby, Aarhus University wrote:
I have used DAKO (20311) anti-s100 to test primary cultures of rat Schwann Cells for purity, I have used the antibody for western blotting (1:1000) and in for immunofluorescence in primary rat Schwann cells. It work very well and is very sensitive for western blotting. Staining also looks correct.
On December 2, 2013 Mads Kjolby wrote:
This antibody works great in western blot on lysates of primary rat Schwann cells. Very specific and sensitive signal/SNR.
Dilution 1:1000.
It also works great for staining of primary rat Schwann cells. The signal looks specific.
Dilution 1:400
On February 19, 2012 Simon Glerup, Aarhus wrote:
Great marker for Schwann cells in immunohistochemistry on cryosection of mouse sciatic nerve. Dilution 1:400
The antibody also works in Western blotting with mouse sciatic nerve homogenates.