This Ab shows a specific band at the expected size of GAD2/GAD65 (65 kDa)
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On May 22, 2017Abdel-Rahman Al-Absi wrote:
5
GAD65, MOUSE MAB, IMMUQUEST #IQ442
I have used this antibody to do western blot on mouse frozen tissue. I have incubated with this antibody (1ug/ml) at 4 °C over night, then with secondary antibody at room temperature for 1 hour. The antibody gives nice band.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On December 18, 2015Simon Mølgaard, Aarhus University wrote:
5
mouse monoclonal Gad65 (IQ442) from ImmuQuest.
This antibody was tested on lysate from adult mouse hippocampus and liver (picture). 50 ug protein was loaded in each lane. manufacturer suggests determining the optimal dilution by titration but recommends starting with 1ug/ml, so for this experiment i used a concentration of 1 ug/ml.
The antibody was left over night at 4°C in a blocking buffer. The secondary antibody was left on for 1 hour at room temperature.
This antibody is excellent for detecting GAD65 by western blotting! A clear band was observed in the hippocampus-sample while nothing was seen in the liver, as expected.
On October 2, 2017 Peter Lund Ovesen wrote:
Mouse hippocampal lysates, 30 ug protein / well.
This Ab shows a specific band at the expected size of GAD2/GAD65 (65 kDa)
On May 22, 2017 Abdel-Rahman Al-Absi wrote:
GAD65, MOUSE MAB, IMMUQUEST #IQ442
I have used this antibody to do western blot on mouse frozen tissue. I have incubated with this antibody (1ug/ml) at 4 °C over night, then with secondary antibody at room temperature for 1 hour. The antibody gives nice band.
On December 18, 2015 Simon Mølgaard, Aarhus University wrote:
mouse monoclonal Gad65 (IQ442) from ImmuQuest.
This antibody was tested on lysate from adult mouse hippocampus and liver (picture). 50 ug protein was loaded in each lane. manufacturer suggests determining the optimal dilution by titration but recommends starting with 1ug/ml, so for this experiment i used a concentration of 1 ug/ml.
The antibody was left over night at 4°C in a blocking buffer. The secondary antibody was left on for 1 hour at room temperature.
This antibody is excellent for detecting GAD65 by western blotting! A clear band was observed in the hippocampus-sample while nothing was seen in the liver, as expected.