On December 18, 2015Simon Mølgaard, Aarhus University wrote:
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Rabbit anti-NMDAR2B (phospho Ser1303) from ImmuQuest, IQ588.
This antibody was tested on lysate from adult mouse hippocampus and liver (picture). 50 ug protein was loaded in each lane. manufacturer suggests determining the optimal dilution by titration but recommends starting with 2ug/ml, so for this experiment i used a concentration of 2 ug/ml.
The antibody was left over night at 4°C in a blocking buffer. The secondary antibody was left on for 1 hour at room temperature.
No bands were observed using this antibody after 20 minutes.
On December 18, 2015 Simon Mølgaard, Aarhus University wrote:
Rabbit anti-NMDAR2B (phospho Ser1303) from ImmuQuest, IQ588.
This antibody was tested on lysate from adult mouse hippocampus and liver (picture). 50 ug protein was loaded in each lane. manufacturer suggests determining the optimal dilution by titration but recommends starting with 2ug/ml, so for this experiment i used a concentration of 2 ug/ml.
The antibody was left over night at 4°C in a blocking buffer. The secondary antibody was left on for 1 hour at room temperature.
No bands were observed using this antibody after 20 minutes.