Related topics: Cellular marker proteins, Ion channels, Neurotransmission
See list of additional phospho-NMDAR2B antibodies at Linscott’s Directory
On December 18, 2015
Simon Mølgaard, Aarhus University wrote:
Rabbit anti-NMDAR2B (phospho Ser1303) from ImmuQuest, IQ588.
This antibody was tested on lysate from adult mouse hippocampus and liver (picture). 50 ug protein was loaded in each lane. manufacturer suggests determining the optimal dilution by titration but recommends starting with 2ug/ml, so for this experiment i used a concentration of 2 ug/ml.
The antibody was left over night at 4°C in a blocking buffer. The secondary antibody was left on for 1 hour at room temperature.
No bands were observed using this antibody after 20 minutes.