Related topics: Cellular marker proteins, Ion channels, Neurotransmission
See list of additional NMDA receptor 1 antibodies at Linscott’s Directory
On December 18, 2015
Simon Mølgaard, Aarhus University wrote:
Rabbit anti-NMDAR1 (IQ591) from ImmuQuest.
This antibody was tested on lysate from adult mouse hippocampus and liver (picture). 50 ug protein was loaded in each lane. manufacturer suggests determining the optimal dilution by titration but recommends starting with 2ug/ml, so for this experiment i used a concentration of 2 ug/ml.
The antibody was left over night at 4°C in a blocking buffer. The secondary antibody was left on for 1 hour at room temperature.
A nice band was observed in the wt sample, while no bands was observed in the liver sample, as expected.