On December 4, 2019Takahito Higashi, The University of Tokyo wrote:
5
This antibody works with immunostaining for identyfing post synaptic densities in a primary hippocampal neuron in a dillution 1:100.
For more information see Higashi et al., Synapse Elimination Triggered by BMP4 Exocytosis and Presynaptic BMP Receptor Activation, Cell Reports, vol 22, issue 4, p919-929, January 23, 2018 (https://doi.org/10.1016/j.celrep.2017.12.101).
Western blot
Not Rated
Immunostaining
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On April 18, 2017Anders Dalby wrote:
1
Did not work for immunostaining mouse hippocampal neurons. Clusters of PSD95 was not visible.
Neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:500 dilution in blocking buffer.
This antibody was tested simultaneously with other antibodies for PSD95 (see below) and proved to be the worst.
PSD95 124 014 (Synaptic Systems) – Guniea pig
PSD95 p246 (Sigma) – Mouse (BEST)
PSD95 MAB 1596 (Chemicon/Millipore) – Mouse
Western blot
Not Rated
Immunostaining
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On February 19, 2012Simon Glerup, Aarhus wrote:
1
Does not work in our hands. We have tested it on synaptosomal preparations from the hippocampus and for staining of primary mouse hippocampal neurons.
On December 4, 2019 Takahito Higashi, The University of Tokyo wrote:
This antibody works with immunostaining for identyfing post synaptic densities in a primary hippocampal neuron in a dillution 1:100.
For more information see Higashi et al., Synapse Elimination Triggered by BMP4 Exocytosis and Presynaptic BMP Receptor Activation, Cell Reports, vol 22, issue 4, p919-929, January 23, 2018 (https://doi.org/10.1016/j.celrep.2017.12.101).
On April 18, 2017 Anders Dalby wrote:
Did not work for immunostaining mouse hippocampal neurons. Clusters of PSD95 was not visible.
Neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:500 dilution in blocking buffer.
This antibody was tested simultaneously with other antibodies for PSD95 (see below) and proved to be the worst.
PSD95 124 014 (Synaptic Systems) – Guniea pig
PSD95 p246 (Sigma) – Mouse (BEST)
PSD95 MAB 1596 (Chemicon/Millipore) – Mouse
On February 19, 2012 Simon Glerup, Aarhus wrote:
Does not work in our hands. We have tested it on synaptosomal preparations from the hippocampus and for staining of primary mouse hippocampal neurons.
IF dilution 1:50
WB 1:1000