Works fine for ICC of hiPSC derived cerebral organoids.
Dilution used: 1:2000
For more information see Ghatak et al. eLife 2019, “Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs. isogenic controls“ https://elifesciences.org/articles/50333v1
I used mouse IgG1 anti human Abeta1-16 (Covance, clone 6E10, # SIG-39320 ) to detect amyloid beta depositions. [note: Covance antibodies has now become BioLegend]
Conditions: 1:500 dilution of antibody in 1% BSA in PBS solution, fixative was ice cold acetone, block solution was DAKO peroxidase blocking reagent.
Tissues: human post mortem cryosections of the cortex of healthy controls, and human AD/CAA and human HCHWA-D patients.
Used DAKO EnVision anti mouse polymer-HRP as a secondary ‘antibody’ treatment.
Results: worked beautifully (just as well as m-a-Abeta clone 4G8, a more regularly used antibody for ABeta detection)
On May 18, 2020 Swagata Ghatak, The Scripps Research Institute wrote:
Works fine for ICC of hiPSC derived cerebral organoids.
Dilution used: 1:2000
For more information see Ghatak et al. eLife 2019, “Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs. isogenic controls“
https://elifesciences.org/articles/50333v1
On December 2, 2015 Maarten Rotman wrote:
I used mouse IgG1 anti human Abeta1-16 (Covance, clone 6E10, # SIG-39320 ) to detect amyloid beta depositions. [note: Covance antibodies has now become BioLegend]
Conditions: 1:500 dilution of antibody in 1% BSA in PBS solution, fixative was ice cold acetone, block solution was DAKO peroxidase blocking reagent.
Tissues: human post mortem cryosections of the cortex of healthy controls, and human AD/CAA and human HCHWA-D patients.
Used DAKO EnVision anti mouse polymer-HRP as a secondary ‘antibody’ treatment.
Results: worked beautifully (just as well as m-a-Abeta clone 4G8, a more regularly used antibody for ABeta detection)
For more info see publication 10.1016/j.neuroscience.2011.05.076 or AlzForum post http://www.alzforum.org/antibodies/app-or-v-amyloid-6e10-0