Does detect the flag epitope, but such high background as to be unusable.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On December 2, 2015Peter Lund Ovesen, Aarhus University wrote:
5
Works in IF to detect FLAG-tagged proteins in Hek293 cells. Great Signal to noise ratio.
This antibody should bind to the same epitope as Sigma’s M2 Anti-FLAG antibody (also reviewed in pAbmAbs)
On June 15, 2020 Bing Zhang initiative wrote:
Does detect the flag epitope, but such high background as to be unusable.
On December 2, 2015 Peter Lund Ovesen, Aarhus University wrote:
Works in IF to detect FLAG-tagged proteins in Hek293 cells. Great Signal to noise ratio.
This antibody should bind to the same epitope as Sigma’s M2 Anti-FLAG antibody (also reviewed in pAbmAbs)
Dilution 1:1000 in 10 % FBS