Antibody works very nicely for ICC. We use rat primary neuronal cultures, PFA fixation and antibody incubation over night at 4°C. 1:500 dilution of recommended reconstitution.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On December 2, 2015Peter Lund Ovesen, Aarhus University wrote:
5
Shank3 guinea pig polyclonal, antiserum (162 304) from Synaptic Systems
This antibody detects postsynaptic-density-like structures along the dendrites of mature primary hippocampal neurons (DIV14).
Neurons were fixed in 4% PFA, permabilized in 0,1 % Triton-X100 and blocked in 10 % FBS
On June 3, 2019 Julia Bär wrote:
Antibody works very nicely for ICC. We use rat primary neuronal cultures, PFA fixation and antibody incubation over night at 4°C. 1:500 dilution of recommended reconstitution.
On December 2, 2015 Peter Lund Ovesen, Aarhus University wrote:
Shank3 guinea pig polyclonal, antiserum (162 304) from Synaptic Systems
This antibody detects postsynaptic-density-like structures along the dendrites of mature primary hippocampal neurons (DIV14).
Neurons were fixed in 4% PFA, permabilized in 0,1 % Triton-X100 and blocked in 10 % FBS
Antibody dilution: 1:500 in 10 % FBS