On January 6, 2017Monara Angelim, INSERM, Paris wrote:
5
I have used it for IHF on brain sections of adult mice, with the dilution 1:100, it worked well, staining cytoplasm. However, I tried it for embryo sections and I do have some staining in cytoplasm, but not for individual cells, well delimitated, as it was with brain sections. Then, i’m not sure of its staining in early developmental stage.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On June 10, 2013Camilla Gustafsen wrote:
5
We have used this antibody for WB detection of proNT3 in inner ear tissue from rat, as well as for detection of human recombinant proNT3. The antibody shows no cross-reactivity with proNGF and does not recognize the mature form of NT3. Please, see Eur J Neurosci. 2011 Feb;33(4):622-31 for further information.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 19, 2012Simon Glerup, Aarhus wrote:
4
The antibody appears to be good and specific for staining proNT3 in PFA-fixed cryosections of mouse embryos. In the developing CNS, only very distinct cell types are stained.
On January 6, 2017 Monara Angelim, INSERM, Paris wrote:
I have used it for IHF on brain sections of adult mice, with the dilution 1:100, it worked well, staining cytoplasm. However, I tried it for embryo sections and I do have some staining in cytoplasm, but not for individual cells, well delimitated, as it was with brain sections. Then, i’m not sure of its staining in early developmental stage.
On June 10, 2013 Camilla Gustafsen wrote:
We have used this antibody for WB detection of proNT3 in inner ear tissue from rat, as well as for detection of human recombinant proNT3. The antibody shows no cross-reactivity with proNGF and does not recognize the mature form of NT3. Please, see Eur J Neurosci. 2011 Feb;33(4):622-31 for further information.
On February 19, 2012 Simon Glerup, Aarhus wrote:
The antibody appears to be good and specific for staining proNT3 in PFA-fixed cryosections of mouse embryos. In the developing CNS, only very distinct cell types are stained.
IF dilution 1:200