GFP, chicken pAb, Abcam, ab13970

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8 User Reviews

  • Works great for ICC on fly brains and labella. Dilution: 1:1000.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • This antibody worked great for immunostaining of perfusion-fixed mice brain slices for IF.
    Dilution used: 1:1000
    For more information see Zha et al. Cell Reports 2020, “VMHvl-Projecting Vglut1+ Neurons in the Posterior Amygdala Gate Territorial Aggression”.
    https://pubmed.ncbi.nlm.nih.gov/32320666/

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Great antibody for immunostaining.

    Dilution used: 1:2000

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Immunostaining were done on 4% paraformaldehyde perfusion-fixed brains that were cut at 50µm on a vibratome.
    Antibody specificity is good and results in robust labeling. Can use relatively dilute concentrations, depending on signal (e.g., 1:2,000 to 1:10,000)

    For more information see Botterill et al. Cell Reports 2019, “An Excitatory and Epileptogenic Effect of Dentate Gyrus Mossy Cells in a Mouse Model of Epilepsy”
    https://www.sciencedirect.com/science/article/pii/S2211124719314330

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Great antibody for IF.
    Dilution used: 1:800

    For more information see Lavery et al. eLife 2020, “ Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
    https://elifesciences.org/articles/52981

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • This antibody worked great for immunostaining.
    Dilution used: 1:500

    For more information see Cui et al. Cell Reports 2019, “Derivation of Mouse Haploid Trophoblast Stem Cells”.
    https://www.sciencedirect.com/science/article/pii/S2211124718320126#

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • This antibody worked great for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.

    Dilution used: 1:1000

    For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).

    Hughes Laboratory:
    https://specciebear.wixsite.com/spinal-cord-hughes

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
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    Flow cytometryNot Rated
    LuminexNot Rated
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  • Anti-GFP Chicken antibody ab13970 Abcam

    We have been using this particular antibody for many years and almost 8 different batches. for immunostainings, never had any complaints and all the batches work the same.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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