I have tested this antibody on both mice brain homogenates as well as in OLN93 and SH-SY5Y cell-lines with primarily poor results. Even addition of Thapsigargin which is known to cause eIF2a phosphorylation only resultet in a weak signal. Used it 1:1000 i 5% BSA.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 12, 2015Julia Abele wrote:
5
Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb 3398
Cell Signaling
On November 23, 2015 Lasse Reimer wrote:
I have tested this antibody on both mice brain homogenates as well as in OLN93 and SH-SY5Y cell-lines with primarily poor results. Even addition of Thapsigargin which is known to cause eIF2a phosphorylation only resultet in a weak signal. Used it 1:1000 i 5% BSA.
On November 12, 2015 Julia Abele wrote:
Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb 3398
Cell Signaling