Related topics: Alphabetical list, Antibody detection, Epitope tags
See list of additional c-myc antibodies at Linscott’s Directory
On March 15, 2016
Sune Skeldal, Aarhus University wrote:
Some success with this antibody in regards to IP of myc-tagged purified proteins (0.5 µg/sample).
Works well in western blotting (1:1000) for detection of purified protein.
On November 6, 2015
Camilla Gustafsen, Aarhus University wrote:
Myc epitope tag
mAb, mouse IgG1
I have used this antibody (1:1000) for WB detection of 1µg Myc-tagged protein. A very strong band was seen. As negative control was used un-tagged protein. I am sure that the antibody can be diluted more, and less protein can be loaded
2. Ab: HRP-anti Mouse IgG, Cell Signaling 7076S (1:2000), 1 hour at RT