This anitbody was tested on lysates from hippocampal neurons either stimulated with BDNF or similar volume of saline added to growth medium. The cells were stimulated for 10 minutes at 37 degrees. lysis buffer containing phosstop and protease inhibitor.
50ug protein was loaded per lane and the antibody was used at a concentration of 1:1000.
This antibody works very well for detecting pMAPK!
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On April 22, 2014Lone Tjener Pallesen, Aarhus University wrote:
5
This antibody is excellent for WB. We stimulated HEK cells transfected with human TrkB with increasing concentrations of BDNF for 10 min. Cells were lysed in buffer containing protease and phosphatase inhibitors. We observed a dose-dependent response to BDNF using this antibody. Two intense bands around 45 kDa were induced by BDNF.
WB dilution 1:1000
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On January 29, 2014Simon Mølgaard wrote:
4
I have also tested this antibody on cultured hippocampal neurons stimulated with 1 nM of BDNF for 10 minutes with great succes. This works very well for detecting phosphorylation of MAPK.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 22, 2012Simon Jensen wrote:
4
This antibody was tested on 293 cells in culture. The cells were stimulated with either 0, 1, 10 or 100nM of insulin before being fixed and stained. This antibody showed clear difference in signal between 0 and 10 nM. This antibody works very well for detecting phosphorylation of Mapk.
Dilution 1:1000
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 19, 2012Anja A, Aarhus wrote:
5
Works great in Western blotting.
We have used it for detecting MAPK phosphorylation upon stimulation of SY5Y cells with GDNF.
WB dilution 1:1000
Diluted antibody can be frozen and reused.
On December 15, 2015 Simon Mølgaard wrote:
This anitbody was tested on lysates from hippocampal neurons either stimulated with BDNF or similar volume of saline added to growth medium. The cells were stimulated for 10 minutes at 37 degrees. lysis buffer containing phosstop and protease inhibitor.
50ug protein was loaded per lane and the antibody was used at a concentration of 1:1000.
This antibody works very well for detecting pMAPK!
On April 22, 2014 Lone Tjener Pallesen, Aarhus University wrote:
This antibody is excellent for WB. We stimulated HEK cells transfected with human TrkB with increasing concentrations of BDNF for 10 min. Cells were lysed in buffer containing protease and phosphatase inhibitors. We observed a dose-dependent response to BDNF using this antibody. Two intense bands around 45 kDa were induced by BDNF.
WB dilution 1:1000
On January 29, 2014 Simon Mølgaard wrote:
I have also tested this antibody on cultured hippocampal neurons stimulated with 1 nM of BDNF for 10 minutes with great succes. This works very well for detecting phosphorylation of MAPK.
On November 22, 2012 Simon Jensen wrote:
This antibody was tested on 293 cells in culture. The cells were stimulated with either 0, 1, 10 or 100nM of insulin before being fixed and stained. This antibody showed clear difference in signal between 0 and 10 nM. This antibody works very well for detecting phosphorylation of Mapk.
Dilution 1:1000
On February 19, 2012 Anja A, Aarhus wrote:
Works great in Western blotting.
We have used it for detecting MAPK phosphorylation upon stimulation of SY5Y cells with GDNF.
WB dilution 1:1000
Diluted antibody can be frozen and reused.