This AB is suppose to detect pro-caspase3 as well as celaved caspase-3. However, I only maneged to detect pro-caspase3 in primary blood purified CD4+ T cells ( treated with stausporine). One fine band for the pro-caspase3, but no band for the celeaved active capse3, therefor only 2 stars. Remeber to block in skim milk.
On October 20, 2015 Sara Konstatin Nissen wrote:
Caspase-3 (8G10) Rabbit mAb #9665 Cell signaling
This AB is suppose to detect pro-caspase3 as well as celaved caspase-3. However, I only maneged to detect pro-caspase3 in primary blood purified CD4+ T cells ( treated with stausporine). One fine band for the pro-caspase3, but no band for the celeaved active capse3, therefor only 2 stars. Remeber to block in skim milk.