phospho=p62 (T269/S272), p196-269, from Phospho Solutions, is not a bad antibody for endogenous detection of phospho=p62. I used at 1:300 in 5%BSA, TBST in 293T cells. For A549 ahd HElla cells, I used this antibody at 1:500 in 5%BSA, TBST. There is not problem when you try to detect protein overexpressed (1:1000, 5% TBST). It does nor work for IP or for IF.
On October 6, 2015 Juan F. Linares wrote:
phospho=p62 (T269/S272), p196-269, from Phospho Solutions, is not a bad antibody for endogenous detection of phospho=p62. I used at 1:300 in 5%BSA, TBST in 293T cells. For A549 ahd HElla cells, I used this antibody at 1:500 in 5%BSA, TBST. There is not problem when you try to detect protein overexpressed (1:1000, 5% TBST). It does nor work for IP or for IF.