GSK3B, mouse mAb, Novus Biologicals # NBP1-47470

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2 User Reviews

  • tested on whole brain cell lysate from mouse, loaded 30 and 10 ug. WB by stainfree gel/membrane, semi-dry blotting, ECL detection.

    Detects 2 bands of the approximate size, one more intense than the other.

    Used dilution 1/5000

    Western blot44444
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • anti-GSK3B, mouse monoclonal, Novus biologicals # NBP1-47470

    Tested for Western blotting on 100 ug total cell lysate from HEK cells, mouse brain and pig brain. used dilution: 1/2000 (recommended)

    In mouse brain, 2 very intense bands of expected size (47 kDa) close to eachother are detected, in pig brain, a fainter band of the same size is detected.
    In HEK cells, e very faint band of a few kDa smaller is detected. GSK3B is not expected to be expressed in kidney (http://www.genecards.org/cgi-bin/carddisp.pl?gene=GSK3B#protein_expression).

    Western blot44444
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex