TRAF6 (sc-7221), from Santa Cruz is a good antibody for WB (1:000, 5% BSA, TBST) and IP (1ug per 1mg pulldown). It does nor works very well in IF. You need amplification with tyramide. I used in 293,HEla, A549 and Pc3 cells.
Western blot
Immunostaining
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On September 4, 2015Simon Glerup, Aarhus University wrote:
1
Traf6 sc7221 from Santa Cruz
Does not work for Western blotting.
Dilution 1:200
RN22 cell lysates were used as samples.
On October 6, 2015 Juan F. Linares wrote:
TRAF6 (sc-7221), from Santa Cruz is a good antibody for WB (1:000, 5% BSA, TBST) and IP (1ug per 1mg pulldown). It does nor works very well in IF. You need amplification with tyramide. I used in 293,HEla, A549 and Pc3 cells.
On September 4, 2015 Simon Glerup, Aarhus University wrote:
Traf6 sc7221 from Santa Cruz
Does not work for Western blotting.
Dilution 1:200
RN22 cell lysates were used as samples.