NeuN, mouse mAb clone A60, Millipore, MAB377

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6 User Reviews

  • This antibody worked roughly for IF (mouse).
    Dilution used: 1:500

    For more information see Süß et al. Cell Reports 2020, “Chronic Peripheral Inflammation Causes a Region-Specific Myeloid Response in the Central Nervous System”.
    https://www.sciencedirect.com/science/article/pii/S2211124720302904

    Western blotNot Rated
    Immunostaining33333
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody works great for immunohistochemistry.
    Dilution used: 1:500

    For more information see Kumamaru et al. Cell Reports 2019, “Regenerating Corticospinal Axons Innervate Phenotypically Appropriate Neurons within Neural Stem Cell Grafts”

    https://www.sciencedirect.com/science/article/pii/S2211124719301366#sec4

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • We used this antibody for IHC staining of mouse brain tissue.
    It works well for both un-perfused (cryosections) and 4% PFA perfused (vibratome sections) tissues.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Works very well for IHC on cryosections of the striatum from PFA fixed mice. Dilution 1:100

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Very good marker of spinal cord dorsal horn neurons as well as dorsal root ganglia neurons (both in IHC and ICC). The IF staining is strong in the nucleus and weaker in the cytoplasm. Dilution: 1:100.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Excellent marker for CNS neurons!

    Recognizes of nuclear protein specific for neurons.

    IF dilution 1:100

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex