On November 1, 2016Andrea E. Toth, Aarhus University wrote:
5
This antibody works very well for immunofluorescence staining in mouse (b.End3) and human (hCMEC/D3) brain endothelial cell line. Dilution 1:200. Fixation was done both with 4% paraformaldehyde or methanol.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On June 29, 2015Piotr Siupka, Aarhus University wrote:
Antibody working very good, giving clear strong signal in primary porcine brain endothelial cells at indicated dilution. For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 488 (1:350) was used. Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.
On November 1, 2016 Andrea E. Toth, Aarhus University wrote:
This antibody works very well for immunofluorescence staining in mouse (b.End3) and human (hCMEC/D3) brain endothelial cell line. Dilution 1:200. Fixation was done both with 4% paraformaldehyde or methanol.
On June 29, 2015 Piotr Siupka, Aarhus University wrote:
Producer: BD Transduction Laboratories
Catalog No: 610133
Used dilution: 1:100 (IF)
Cell line: primary porcine brain endothelial cells
Host organism: Mouse
Character: primary, monoclonal
Antibody working very good, giving clear strong signal in primary porcine brain endothelial cells at indicated dilution. For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 488 (1:350) was used. Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.