Antibody working nice at indicated dilution. Tested in primary porcine brain endothelial cells. For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 568 (1:350) was used. Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.
On June 29, 2015 Piotr Siupka, Aarhus University wrote:
Producer: Invitrogen
Catalog No: 617 300
Used dilution: 1:100 (IF)
Cell line: primary porcine brain endothelial cells
Host organism: Rabbit
Character: primary, polyclonal
Antibody working nice at indicated dilution. Tested in primary porcine brain endothelial cells. For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 568 (1:350) was used. Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.