Antibody tested in primary porcine and bovine brain endothelial cells. Working good at indicated dilution. For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 568 (1:350) was used.
Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.
Please see images above.
On June 29, 2015 Piotr Siupka, Aarhus University wrote:
Producer: Abcam
Catalog No: ab53765
Used dilution: 1:100 (IF)
Cell line: primary porcine brain endothelial cells, primary bovine brain endothelial cells, MDCK
Host organism: Rabbit
Character: primary, polyclonal
Antibody tested in primary porcine and bovine brain endothelial cells. Working good at indicated dilution. For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 568 (1:350) was used.
Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.
Please see images above.