bovine MPR46, mouse mAb, Developmental Studies Hybridoma Bank, 22d4

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  • Producer: Developmental Studies Hybridoma Bank
    Catalog No: 22d4
    Used dilution: 1:100 (IF)
    Cell line: primary bovine brain endothelial cells
    Host organism: Mouse
    Character: primary, monoclonal

    Antibody tested in primary bovine brain endothelial cells. Works very nicely at indicated dilution, giving clear staining. Antibody raised against bovine CD-M6PR. Other dilutions not tested so far nor WB. For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 488 (1:350) was used.
    Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.

    Western blotNot Rated
    ImmunostainingNot Rated
    IP33333
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex
EM