bovine MPR300, mouse mAb, Developmental Studies Hybridoma Bank, 86f7

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  • Producer: Developmental Studies Hybridoma Bank
    Catalog No: 86f7
    Used dilution: 1:100; 1:200; 1:300 (IF); 1:100 (WB)
    Cell line: primary bovine brain endothelial cells, primary porcine brain endothelial cells
    Host organism: Ms
    Character: primary, monoclonal

    Antibody tested for IF and WB. Raised against bovine CI-M6PR works nicely in primary bovine brain endothelial cells in mentioned dilutions (most often 1:100 used). Both WB and IF looks good.
    For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 488 (1:350) was used.

    Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.

    Western blotNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Flow cytometry