For more information see Lavery et al. eLife 2020, “ Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome https://elifesciences.org/articles/52981
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On May 19, 2017Veerle Paternoster wrote:
5
Histone H3, Rabbit, Abcam #ab1791
Excellent antibody for Western blotting. Works both on histone extracts (acid extraction, mouse brain) as cell lysates (RIPA buffer, mouse brain).
C-terminal epitope, so can detect clipped histone H3 as well.
On June 16, 2020 Bing Zhang initiative wrote:
Used for ICC on fly embryo at the dilution of 1:1000.
1ug used for CO-IP.
Used for WB at the dilution of 1:20.000.
On April 24, 2020 Huda Zoghbi, Baylor College of Medicine wrote:
Worked fine for WB.
Dilution used: 1:20.000
For more information see Lavery et al. eLife 2020, “ Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
https://elifesciences.org/articles/52981
On May 19, 2017 Veerle Paternoster wrote:
Histone H3, Rabbit, Abcam #ab1791
Excellent antibody for Western blotting. Works both on histone extracts (acid extraction, mouse brain) as cell lysates (RIPA buffer, mouse brain).
C-terminal epitope, so can detect clipped histone H3 as well.
WB details: BIS-TRIS gel+XT-MES running buffer, blocked in TBS+5%BSA, 1:20 000 dilution.
On June 16, 2015 Philip Yung, Cavalli lab, CNRS wrote:
Not working for immunostaining of Drosophila Imaginal Discs