Related topics: Alphabetical list, Metabolism, Signal transduction
See list of additional Phospho-GSK-3beta antibodies at Linscott’s Directory
On November 25, 2019
Giorgio Ramadori, University of Geneva, Department of Cellular Physiology and Metabolism wrote:
We have used this antibody for western blotting with great succes.
For more information see Aras et al., 2019, Cell Reports 27, 2385–2398 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30573-X)
On January 24, 2017
Marko Rosenholm wrote:
Phospho-GSK-3β (Ser9) Antibody #9336
Cell Signaling Technology
This antibody has regularly given a clear and specific band at expected molecular weight when detecting GSK3β phosphorylation in mouse hippocampal and cortical lysates in Western blot.
The antibody is diluted 1:1000 in 3% BSA in TBS-T + 1:1000 NaN3. Dilution has given a good signal for 5-7 uses when stored at 4 degrees.
Incubation overnight at 4 degrees
Washing steps with TBS-T
Secondary antibody diluted in 5% milk powder in TBS-T for 1 hour
Detection with ECL+ (ThermoScientific)
On June 8, 2015
Mads Kjolby, Aarhus University wrote:
I have used anti-phosphoGK3beta Ser9 (CST #9336) at 1:1000 on murine heart tissue. Gives a nice band at 45 kDa as expected.