On June 8, 2015Juan Yuan, Aarhus University wrote:
5
This antibody is excellent to be chosen as an internal control for western blot. I have tested both in neural progenitor cell and medulloblastoma cell. Both cell line the signal is very clear, sharp, specific, no background.
Primary antibody: 1:1000 dilution in 5%milk/TBST buffer, incubate in RT for 1 hour.
Secondary antibody: anti-mouse IgG-HRP, 1:10000 dilution in 5%milk/TBST buffer, incubate inRT for 1 hour.
Image the signal using ImageQuant for exposure time of 1-3 sec.
See image above.
On June 8, 2015 Juan Yuan, Aarhus University wrote:
This antibody is excellent to be chosen as an internal control for western blot. I have tested both in neural progenitor cell and medulloblastoma cell. Both cell line the signal is very clear, sharp, specific, no background.
Primary antibody: 1:1000 dilution in 5%milk/TBST buffer, incubate in RT for 1 hour.
Secondary antibody: anti-mouse IgG-HRP, 1:10000 dilution in 5%milk/TBST buffer, incubate inRT for 1 hour.
Image the signal using ImageQuant for exposure time of 1-3 sec.
See image above.