On May 10, 2015Jan Herudek (Aarhus University) wrote:
2
Rabbit polyclonal detects 34 kDa ZC3H14 protein. Tested in whole cell extract (HeLa cells). Working dilution for western blotting 1:1000 in 5% milk/PBST and overnight incubation in cold room. Antibody provides pretty high nonspecific background. Dilution of secondary antibody 1:5000 in PBST.
On May 10, 2015 Jan Herudek (Aarhus University) wrote:
Rabbit polyclonal detects 34 kDa ZC3H14 protein. Tested in whole cell extract (HeLa cells). Working dilution for western blotting 1:1000 in 5% milk/PBST and overnight incubation in cold room. Antibody provides pretty high nonspecific background. Dilution of secondary antibody 1:5000 in PBST.