Related topics: Transcriptional regulation
See list of additional Pol II antibodies at Linscott’s Directory
On May 10, 2015 Pia Andersen, Aarhus University wrote:
Stock solution: 200ug/0.1ml
Used for ChIP in Hek-293 Flip-in T-Rex cells and in HeLa cells Gives nice signal when using protein A coupled dynabeads
Pr IP 2.5ul of stock solution is used. One IP is done on material from half a P10 plate which is approx. 80% confluent for both cell types.
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On May 10, 2015 Pia Andersen, Aarhus University wrote:
Stock solution: 200ug/0.1ml
Used for ChIP in Hek-293 Flip-in T-Rex cells and in HeLa cells
Gives nice signal when using protein A coupled dynabeads
Pr IP 2.5ul of stock solution is used. One IP is done on material from half a P10 plate which is approx. 80% confluent for both cell types.