On May 10, 2015Pia Andersen, Aarhus University wrote:
1
Stock solution: 200ug/0.1ml
Used for ChIP in Hek-293 Flip-in T-Rex cells and in HeLa cells
Where not able to get any signal over background when using protein A coupled agarose beads
Pr IP 5 ul of stock solution is used. One IP is done on material from half a P10 plate which is approx. 80% confluent for both cell types.
On May 10, 2015 Pia Andersen, Aarhus University wrote:
Stock solution: 200ug/0.1ml
Used for ChIP in Hek-293 Flip-in T-Rex cells and in HeLa cells
Where not able to get any signal over background when using protein A coupled agarose beads
Pr IP 5 ul of stock solution is used. One IP is done on material from half a P10 plate which is approx. 80% confluent for both cell types.