Parvalbumin, mouse mAb clone PARV-19, Millipore, MAB1572

  • 44444
  • 3.53.53.53.53.5
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2 User Reviews

  • Did not work very well for ICC of hiPSC derived neuronal cultures.

    Dilution used: 1:100

    For more information see Ghatak et al. eLife 2019, “Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs. isogenic controls“
    https://elifesciences.org/articles/50333v1

    Western blotNot Rated
    Immunostaining22222
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Protocols used: DAB (1:2000), immunofluorescence (1:500 – 1:1,1000), Western blot (1:500)
    Species: mouse, human

    This antibody works extremely well for immunofluorescence and DAB staining in mouse and human fixed brain sections, giving high signal and low noise for both species. For Western blot, this antibody gives a single band at the specified molecular weight, but the low weight of this protein made the protocol inherently tricky.

    References:
    Lucas, E.K., Reid, C.S., McMeekin, L.J., Dougherty, S.E., & Cowell, R.M. (2015). Cerebellar transcriptional alterations with Purkinje cell dysfunction and loss in mice lacking PGC-1α. Frontiers in Cellular Neuroscience, Special Issue, Neurodegeneration: from Genetics to Molecules, 8, 441:1-13.

    Lucas, E.K., Dougherty, S.E., McMeekin, L.J., Reid, C.S., West, A.B., Dobrunz, L.E., Hablitz, J.J., & Cowell, R.M. (2014). PGC-1α provides a transcriptional framework for synchronous neurotransmitter release from parvalbumin-positive interneurons. Journal of Neuroscience, 34, 43: 14375-14387.

    Lucas, E.K., Jegarl, A., & Clem, R.L. (2014). Mice lacking TrkB in parvalbumin-positive cells exhibit sexually dimorphic behavioral phenotypes. Behavioural Brain Research, 274: 219-225.

    Dougherty, S.E., Reeves, J. L., Lucas, E. K., Lesort, M. J., Detloff, P. J. Gamble, K. L., & Cowell, R. M. (2012). Presymptomatic abnormalities in Purkinje cell function and viability in animal models of HD. Experimental Neurology, 236: 171-178.

    Lucas, E.K., Markwardt, S.J., Gupta, S., Meador-Woodruff, J.H., Lin, J.D., Overstreet-Wadiche, L., & Cowell, R.M. (2010). Parvalbumin deficiency and GABAergic dysfunction in mice lacking PGC-1α. Journal of Neuroscience, 30, 21, 7227-7235.

    Western blot44444
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM