Did not work very well for ICC of hiPSC derived neuronal cultures.
Dilution used: 1:100
For more information see Ghatak et al. eLife 2019, “Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs. isogenic controls“ https://elifesciences.org/articles/50333v1
Western blot
Not Rated
Immunostaining
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On May 8, 2015Elizabeth Lucas, Mount Sinai Hospital wrote:
4.5
Protocols used: DAB (1:2000), immunofluorescence (1:500 – 1:1,1000), Western blot (1:500)
Species: mouse, human
This antibody works extremely well for immunofluorescence and DAB staining in mouse and human fixed brain sections, giving high signal and low noise for both species. For Western blot, this antibody gives a single band at the specified molecular weight, but the low weight of this protein made the protocol inherently tricky.
References:
Lucas, E.K., Reid, C.S., McMeekin, L.J., Dougherty, S.E., & Cowell, R.M. (2015). Cerebellar transcriptional alterations with Purkinje cell dysfunction and loss in mice lacking PGC-1α. Frontiers in Cellular Neuroscience, Special Issue, Neurodegeneration: from Genetics to Molecules, 8, 441:1-13.
Lucas, E.K., Dougherty, S.E., McMeekin, L.J., Reid, C.S., West, A.B., Dobrunz, L.E., Hablitz, J.J., & Cowell, R.M. (2014). PGC-1α provides a transcriptional framework for synchronous neurotransmitter release from parvalbumin-positive interneurons. Journal of Neuroscience, 34, 43: 14375-14387.
Dougherty, S.E., Reeves, J. L., Lucas, E. K., Lesort, M. J., Detloff, P. J. Gamble, K. L., & Cowell, R. M. (2012). Presymptomatic abnormalities in Purkinje cell function and viability in animal models of HD. Experimental Neurology, 236: 171-178.
On May 18, 2020 Swagata Ghatak, The Scripps Research Institute wrote:
Did not work very well for ICC of hiPSC derived neuronal cultures.
Dilution used: 1:100
For more information see Ghatak et al. eLife 2019, “Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs. isogenic controls“
https://elifesciences.org/articles/50333v1
On May 8, 2015 Elizabeth Lucas, Mount Sinai Hospital wrote:
Protocols used: DAB (1:2000), immunofluorescence (1:500 – 1:1,1000), Western blot (1:500)
Species: mouse, human
This antibody works extremely well for immunofluorescence and DAB staining in mouse and human fixed brain sections, giving high signal and low noise for both species. For Western blot, this antibody gives a single band at the specified molecular weight, but the low weight of this protein made the protocol inherently tricky.
References:
Lucas, E.K., Reid, C.S., McMeekin, L.J., Dougherty, S.E., & Cowell, R.M. (2015). Cerebellar transcriptional alterations with Purkinje cell dysfunction and loss in mice lacking PGC-1α. Frontiers in Cellular Neuroscience, Special Issue, Neurodegeneration: from Genetics to Molecules, 8, 441:1-13.
Lucas, E.K., Dougherty, S.E., McMeekin, L.J., Reid, C.S., West, A.B., Dobrunz, L.E., Hablitz, J.J., & Cowell, R.M. (2014). PGC-1α provides a transcriptional framework for synchronous neurotransmitter release from parvalbumin-positive interneurons. Journal of Neuroscience, 34, 43: 14375-14387.
Lucas, E.K., Jegarl, A., & Clem, R.L. (2014). Mice lacking TrkB in parvalbumin-positive cells exhibit sexually dimorphic behavioral phenotypes. Behavioural Brain Research, 274: 219-225.
Dougherty, S.E., Reeves, J. L., Lucas, E. K., Lesort, M. J., Detloff, P. J. Gamble, K. L., & Cowell, R. M. (2012). Presymptomatic abnormalities in Purkinje cell function and viability in animal models of HD. Experimental Neurology, 236: 171-178.
Lucas, E.K., Markwardt, S.J., Gupta, S., Meador-Woodruff, J.H., Lin, J.D., Overstreet-Wadiche, L., & Cowell, R.M. (2010). Parvalbumin deficiency and GABAergic dysfunction in mice lacking PGC-1α. Journal of Neuroscience, 30, 21, 7227-7235.