GABA receptor subtype alpha 2, mouse mAb IgM, Synaptic systems # 224 111

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One User Review

  • This antibody was tested on 15 um thick hippocampal cryo sections from a perfusion fixed mouse. Prior to immunohistochemistry, the tissue was target retrieved using DAKO antigen retrieval s1699.

    NOTE: This antibody is IgM and was therefore tested using goat anti-mouse IgM 568 (#A-21043) from Life Technologies.

    The antibody did not work for IHC. No signal was observed but a somewhat high background was seen. The manufacturer suggested treating the tissue with pepsin due to the size of the IgM molecule. This however, i did not try.

    Western blotNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
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