DDX6, rabbit pAb, Bethyl Laboratories, A300-461A

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One User Review

  • Western using lysates from HeLa, NHDF, HEK293
    1:5000 for western (standard 5% milk, TBS-T buffer).
    Secondary antibodies dilution: 1:5.000 of goat anti-rabbit/HRP antibodies in 5%milk/TBS-T (DAKO #P0448) (incubation time: 1hr at RT)
    Chemiluminescent substrate: SuperSignal West Femto (Thermo Scientific #34096)

    Immunofluorescence using HeLa, NHDF, HEK293 cells fixed in 4% PFA (PBS), Permeabilization in 0.5% Triton/PBS/1% horse serum). Primary: 1:1000 dilution in PBS/1% horse serum. Secondary: 1:1000 dilution PBS/1% horse serum. Donkey anti rabbit – Alexa 488 or Alexa 594 conjugated. Processing body staining.

    Western blot55555
    Immunostaining44444
    IP44444
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM