GABA(A) receptor subtype alpha 1, rabbit pAb, Synaptic systems # 224 203

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2 User Reviews

  • This antibody works great to detect GABA-A receptor subunit alpha 1 in lysates from murine primary neurons (cortical). A very short exposure time were needed to develop bands using this antibody.

    Dilution 1:1000

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • This antibody was tested on 15 um thick hippocampal cryo sections from a perfusion fixed mouse. Prior to immunohistochemistry, the tissue was target retrieved using DAKO antigen retrieval s1699 according to protocol from the manufacturer.

    This antibody works excellent for detecting expression of GABA(A) receptor subtype alpha 1 in the hippocampus.

    This was tested at 1:500 and1:1000. At 1:1000 a clear signal was seen with low background. The manufacturer suggest using a dilution between 1:1000 to 1:5000 so an even better result may be obtained using 1:5000.

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex
EM