This antibody works very well in western blotting.
For more information see Mymrikov et al., The Chaperone Activity and Substrate Spectrum of Human Small Heat Shock Proteins, The Journal of Biological Chemistry, November 30, 2016 (http://www.jbc.org/content/292/2/672.long).
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On December 13, 2018Kasper Budolph Pedersen wrote:
5
Excellent control for HepG2 cell lysates. Reused +25 times and diluted 1:5000
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On June 22, 2017Camilla Gustafsen, Aarhus Universitet wrote:
5
Great loading control for HepG2 cell lysates. Single band detected at 38 kDa marker band (See blue Plus2 marker). We dilute at least 1:2000 and re-use several times.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On August 27, 2015Simon Mølgaard wrote:
5
This was tested on lysates from hippocampal tissue. Works excellent for detection of GAPDH! Also works for reprobing stripped membranes. Was used at a dilution of 1:5000
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On November 24, 2014Melissa Wren wrote:
5
Superior antibody to probe as this house keeping gene is less susceptible to modulation by extraneous variable (except changes in mitochondrial function) whereas other common loading controls such as actin and tubulin can be altered by many different variables. I have used this antibody on cell lysates from primary cortical neuronal cultures and motor neuron cultures from nice, also human iPSC derived cells and human ES cells. Works great. 1:5000 for WB, more than enough for 2H at room temperature.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On August 6, 2013Camilla Gustafsen wrote:
0
This antibody is not good for Western blotting of lysates obtained from cross-linked (DSP) HEK cells
On February 13, 2012Simon Glerup wrote:
5
Excellent for Western blotting 1:5000
Can also be used for reprobing stripped membranes.
Superb as loading control. May be superior to beta-actin.
Beta-actin levels are affected dramatically by e.g. insulin-induced signaling cascades.
On September 24, 2019 Evgeny Mymrikov, Institute of Biochemistry and Molecular Biology, University of Freiburg wrote:
This antibody works very well in western blotting.
For more information see Mymrikov et al., The Chaperone Activity and Substrate Spectrum of Human Small Heat Shock Proteins, The Journal of Biological Chemistry, November 30, 2016 (http://www.jbc.org/content/292/2/672.long).
On December 13, 2018 Kasper Budolph Pedersen wrote:
Excellent control for HepG2 cell lysates. Reused +25 times and diluted 1:5000
On June 22, 2017 Camilla Gustafsen, Aarhus Universitet wrote:
Great loading control for HepG2 cell lysates. Single band detected at 38 kDa marker band (See blue Plus2 marker). We dilute at least 1:2000 and re-use several times.
On August 27, 2015 Simon Mølgaard wrote:
This was tested on lysates from hippocampal tissue. Works excellent for detection of GAPDH! Also works for reprobing stripped membranes. Was used at a dilution of 1:5000
On November 24, 2014 Melissa Wren wrote:
Superior antibody to probe as this house keeping gene is less susceptible to modulation by extraneous variable (except changes in mitochondrial function) whereas other common loading controls such as actin and tubulin can be altered by many different variables. I have used this antibody on cell lysates from primary cortical neuronal cultures and motor neuron cultures from nice, also human iPSC derived cells and human ES cells. Works great. 1:5000 for WB, more than enough for 2H at room temperature.
On August 6, 2013 Camilla Gustafsen wrote:
This antibody is not good for Western blotting of lysates obtained from cross-linked (DSP) HEK cells
On February 13, 2012 Simon Glerup wrote:
Excellent for Western blotting 1:5000
Can also be used for reprobing stripped membranes.
Superb as loading control. May be superior to beta-actin.
Beta-actin levels are affected dramatically by e.g. insulin-induced signaling cascades.