Related topics: Immunology
See list of additional HA antibodies at Linscott’s Directory
On March 15, 2016
Sune Skeldal, Aarhus University wrote:
This rabbit anti-HA-tag antibody is perfect for western blotting (1:1000). Detected tagged proteins from HEK293 cell lysate. Only a faint high molecular band appears as background staining.
On January 8, 2015
Dennis Berg Holt, Aarhus University wrote:
1:1000 dilution used, incubation over night at 4°C. Samples: ‘Dirty’ total protein extracts of plant roots (Lotus japonicus).
Worked well. Only a single (faint) unspecific band. I went on to use this antibody in a ChIP-seq experiment, but I haven’t got the results for that experiment yet.