Related topics: Alphabetical list, Drosophila, Epitope tags
See list of additional FLAG antibodies at Linscott’s Directory
On June 15, 2020
Bing Zhang initiative wrote:
Does detect the flag epitope, but such high background as to be unusable.
On May 5, 2020
Margie Sutton, University of Texas MD Anderson Cancer Center wrote:
WB dilution 1:5000
For more information see Sutton et al. Cell Reports 2019, “DIRAS3 (ARHI) Blocks RAS/MAPK Signaling by Binding Directly to RAS and Disrupting RAS Clusters”
On February 29, 2016
Rachel L. Flynn, Boston University School of Medicine, US wrote:
This antibody was used for ChIP (2µg).
Reference K. E. Cox et al., Cell Reports, 9 February 2016
On January 29, 2016
Cinzia Tesauro, Aarhus University wrote:
I have used this antibody for many years to verify the expression and purity of flag-tagged proteins. Never had problems of background.
On January 16, 2015
Jakob Vejby Larsen wrote:
I have used this antibody in 293 cells transfected with a protein containing a FLAG-tag in the N-terminus. It works great in both WB and IF (dilution 1:1000)
On January 13, 2015
Kasper Mølgaard Jensen wrote:
anti-FLAG® M2 mouse mAb Sigma-Aldrich F1804
I have used anti-FLAG in western blotting to detect bispecific diabodies (along with anti-myc), and it works perfectly. Low background and very specific detection of FLAG taged antibodies was observed. Furthermore it was possible to detect a difference in expression levels of the two chains of the diabody.
Used as 1:4000 [0.25ug/mL].
On January 8, 2015
Kasper Mølgaard Jensen, Aarhus University wrote:
I have used this in ELISA and have had great results, very low background and high specificity. Used in concentration of 0.2ug/mL.
On January 8, 2015
Bjarke Primdal-Bengtson, Aarhus University wrote:
I have used this with great succes for quantifying expression of FLAG-tagged Rab27B using Western blot. Also, I have looked upon binding partners to FLAG-tagged Rab27B through IP.