On February 29, 2016Rachel L. Flynn, Boston University School of Medicine, US wrote:
5
This antibody was used for ChIP (2µg).
Reference K. E. Cox et al., Cell Reports, 9 February 2016
Western blot
Not Rated
Immunostaining
Not Rated
IP
Not Rated
ChIP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On January 29, 2016Cinzia Tesauro, Aarhus University wrote:
5
I have used this antibody for many years to verify the expression and purity of flag-tagged proteins. Never had problems of background.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On January 16, 2015Jakob Vejby Larsen wrote:
5
I have used this antibody in 293 cells transfected with a protein containing a FLAG-tag in the N-terminus. It works great in both WB and IF (dilution 1:1000)
Western blot
Immunostaining
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On January 13, 2015Kasper Mølgaard Jensen wrote:
5
anti-FLAG® M2 mouse mAb Sigma-Aldrich F1804
I have used anti-FLAG in western blotting to detect bispecific diabodies (along with anti-myc), and it works perfectly. Low background and very specific detection of FLAG taged antibodies was observed. Furthermore it was possible to detect a difference in expression levels of the two chains of the diabody.
Used as 1:4000 [0.25ug/mL].
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On January 8, 2015Kasper Mølgaard Jensen, Aarhus University wrote:
5
I have used this in ELISA and have had great results, very low background and high specificity. Used in concentration of 0.2ug/mL.
Western blot
Not Rated
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On January 8, 2015Bjarke Primdal-Bengtson, Aarhus University wrote:
5
I have used this with great succes for quantifying expression of FLAG-tagged Rab27B using Western blot. Also, I have looked upon binding partners to FLAG-tagged Rab27B through IP.
On August 20, 2022 Wang, Chinese Academy of Sciences wrote:
Dilution 1:500 for immunostaining
On June 15, 2020 Bing Zhang initiative wrote:
Does detect the flag epitope, but such high background as to be unusable.
On May 5, 2020 Margie Sutton, University of Texas MD Anderson Cancer Center wrote:
WB dilution 1:5000
For more information see Sutton et al. Cell Reports 2019, “DIRAS3 (ARHI) Blocks RAS/MAPK Signaling by Binding Directly to RAS and Disrupting RAS Clusters”
https://www.sciencedirect.com/science/article/pii/S221112471931527X#sec5
On February 29, 2016 Rachel L. Flynn, Boston University School of Medicine, US wrote:
This antibody was used for ChIP (2µg).
Reference K. E. Cox et al., Cell Reports, 9 February 2016
On January 29, 2016 Cinzia Tesauro, Aarhus University wrote:
I have used this antibody for many years to verify the expression and purity of flag-tagged proteins. Never had problems of background.
On January 16, 2015 Jakob Vejby Larsen wrote:
I have used this antibody in 293 cells transfected with a protein containing a FLAG-tag in the N-terminus. It works great in both WB and IF (dilution 1:1000)
On January 13, 2015 Kasper Mølgaard Jensen wrote:
anti-FLAG® M2 mouse mAb Sigma-Aldrich F1804
I have used anti-FLAG in western blotting to detect bispecific diabodies (along with anti-myc), and it works perfectly. Low background and very specific detection of FLAG taged antibodies was observed. Furthermore it was possible to detect a difference in expression levels of the two chains of the diabody.
Used as 1:4000 [0.25ug/mL].
On January 8, 2015 Kasper Mølgaard Jensen, Aarhus University wrote:
I have used this in ELISA and have had great results, very low background and high specificity. Used in concentration of 0.2ug/mL.
On January 8, 2015 Bjarke Primdal-Bengtson, Aarhus University wrote:
I have used this with great succes for quantifying expression of FLAG-tagged Rab27B using Western blot. Also, I have looked upon binding partners to FLAG-tagged Rab27B through IP.