FLAG, mouse mAb clone M2, Sigma-Aldrich, F1804

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8 User Reviews

  • Does detect the flag epitope, but such high background as to be unusable.

    Western blot11111
    ImmunostainingNot Rated
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  • WB dilution 1:5000

    For more information see Sutton et al. Cell Reports 2019, “DIRAS3 (ARHI) Blocks RAS/MAPK Signaling by Binding Directly to RAS and Disrupting RAS Clusters”
    https://www.sciencedirect.com/science/article/pii/S221112471931527X#sec5

    Western blot55555
    Immunostaining55555
    IP55555
    ChIPNot Rated
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  • This antibody was used for ChIP (2µg).
    Reference K. E. Cox et al., Cell Reports, 9 February 2016

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  • I have used this antibody for many years to verify the expression and purity of flag-tagged proteins. Never had problems of background.

    Western blot55555
    ImmunostainingNot Rated
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  • I have used this antibody in 293 cells transfected with a protein containing a FLAG-tag in the N-terminus. It works great in both WB and IF (dilution 1:1000)

    Western blot55555
    Immunostaining55555
    IPNot Rated
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  • anti-FLAG® M2 mouse mAb Sigma-Aldrich F1804
    I have used anti-FLAG in western blotting to detect bispecific diabodies (along with anti-myc), and it works perfectly. Low background and very specific detection of FLAG taged antibodies was observed. Furthermore it was possible to detect a difference in expression levels of the two chains of the diabody.
    Used as 1:4000 [0.25ug/mL].

    Western blot55555
    ImmunostainingNot Rated
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  • I have used this in ELISA and have had great results, very low background and high specificity. Used in concentration of 0.2ug/mL.

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    ELISA55555
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  • I have used this with great succes for quantifying expression of FLAG-tagged Rab27B using Western blot. Also, I have looked upon binding partners to FLAG-tagged Rab27B through IP.

    Western blot55555
    ImmunostainingNot Rated
    IP55555
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM