On January 8, 2015Peter Lund Ovesen, Aarhus University wrote:
3.5
I had succes using this in immunostainings of HEK293 cells transient transfected with human Neurexin-1-beta cDNA. (Dilution 1:100)
For western blotting, we had difficulties detecting Neurexin-1 in lysates of both tranfected 293 cells and mouse brain. Though, we see positive staining in a lane containing 20ng recombinant human Neurexin-1-beta. (Dilution 1:500)
Western blot
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On January 8, 2015Peter Lund Ovesen, Aarhus University wrote:
3.5
Neurexin-1 goat polyclonal antibody (P-15) #sc-14334 from Santa cruz
I had succes using this in immunostainings of HEK293 cells transient transfected with human Neurexin-1-beta cDNA. (Dilution 1:100)
For western blotting, we had difficulties detecting Neurexin-1 in lysates of both tranfected 293 cells and mouse brain. Though, we see positive staining in a lane containing 20ng recombinant human Neurexin-1-beta. (Dilution 1:500)
On January 8, 2015 Peter Lund Ovesen, Aarhus University wrote:
I had succes using this in immunostainings of HEK293 cells transient transfected with human Neurexin-1-beta cDNA. (Dilution 1:100)
For western blotting, we had difficulties detecting Neurexin-1 in lysates of both tranfected 293 cells and mouse brain. Though, we see positive staining in a lane containing 20ng recombinant human Neurexin-1-beta. (Dilution 1:500)
On January 8, 2015 Peter Lund Ovesen, Aarhus University wrote:
Neurexin-1 goat polyclonal antibody (P-15) #sc-14334 from Santa cruz
I had succes using this in immunostainings of HEK293 cells transient transfected with human Neurexin-1-beta cDNA. (Dilution 1:100)
For western blotting, we had difficulties detecting Neurexin-1 in lysates of both tranfected 293 cells and mouse brain. Though, we see positive staining in a lane containing 20ng recombinant human Neurexin-1-beta. (Dilution 1:500)